精料类型对肉羊饲粮养分消化率及尿嘌呤衍生物排出量的影响

doi:10.11843/j.issn.0366-6964.2018.07.008

Abstract

Abstract:

The aim of this study was to establish a model for estimating microbial nitrogen with purine derivatives. Ten Dorper×thin-tailed Han crossbred, non-castrated adult rams with(47.4±4.4) kg BW were assigned to 10 experimental diet treatments with different concentrate sources in a single factor design, and 10 rams in each treatment. The experimental diet included 10 pelleted TMR with a single concentrate ratio of 30%. The experiment included 10 periods in which 10 lambs were fed with one of the 10 pelleted TMR. Each period lasted for 20 d(15 d for adaptation and 5 d for trial period) and the total experiment lasted for 200 d. This experiment study the effect of 10 different concentrates on nutrient digestibility and excretion of purine derivatives in urine of mutton sheep. The results showed that:1)The digestibility of nutrients in diets was related to the content of nutrients, and the digestibility of diets with different concentrates was different. The best predictors of dietary digestible crude protein(DCP) was crude protein(CP). The urinary nitrogen, fecal nitrogen and intake nitrogen of lambs fed 10 diets with different concentrates showed significant difference(P<0.01). 2) The proportion of allantoin, uric acid, xanthine(included hypoxanthine) in total purine derivative(PD) excretion were significantly different(P<0.01) among 10 treatments, and the ratios were 85.60%-92.47%, 2.54%-7.18%, 3.20%-7.41%, respectively. Purine nitrogen indexes were significantly different(P<0.01) among 10 treatments, and range was 0.03-0.10. However, PD excretion had a strong linear relationship with intake nitrogen and microbial nitrogen(MN). Intake N had a strong relationship with apparent digestibility of N, urine nitrogen and retained N. A linear relationship was found between retained N(Y) and intake N:Y=0.244 9×intake N-1.319 9(R²=0.825). A linear relationship was found between PD excretion and intake N:PD excretion=0.119 7×intake N+10.161(R²=0.925), and a linear relationship was found between PD excretion and MN:MN=0.746 1×PD+1.785 4(R²=0.898).

CLC Number:

FU Li-xia, MA Tao, DIAO Qi-yu, CHENG Shu-ru, SONG Ya-zhe. Effect of Different Concentrates on Nutrient Digestibility and Excretion of Purine Derivatives in Urine of Mutton Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1394-1404.

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References

[1] AFRC.Technical committee on responses to nutrients,Report 9.Nutritive requirements of ruminant animals:protein[J].Nutr Abs Rev, Series B,1992,62:787-835.
[2] MARTÍNORÚE S M,BALCELLS J,GUADA J A,et al.Microbial nitrogen production in growing heifers:direct measurement of duodenal flow of purine bases versus urinary excretion of purine derivatives as estimation procedures[J].Anim Feed Sci Technol,2000,88(3-4):171-188.
[3] GONZALEZ-RONQUILLO M,BALCELLS J,GUADA J A,et al.Purine derivative excretion in dairy cows:endogenous excretion and the effect of exogenous nucleic acid supply[J].J Dairy Sci,2003,86(4):1282-1291.
[4] MA T,DENG K D,JIANG C G,et al.The relationship between microbial N synthesis and urinary excretion of purine derivatives in Dorper×thin-tailed Han crossbred sheep[J].Small Rumin Res,2013,112(1-3):49-55.
[5] MA T,DENG K D,TU Y,et al.Effect of dietary forage-to-concentrate ratios on urinary excretion of purine derivatives and microbial nitrogen yields in the rumen of Dorper crossbred sheep[J].Livest Sci,2014,160:37-44.
[6] 马涛,刁其玉,邓凯东,等.饲粮不同采食水平下肉羊氮沉积和尿中嘌呤衍生物排出规律的研究[J].动物营养学报,2012,24(7):1229-1235.
MA T,DIAO Q Y,DENG K D,et al.Nitrogen retention and urinary excretion of purine derivatives of mutton sheep at different levels of feed intake[J].Chinese Journal of Animal Nutrition,2012,24(7):1229-1235. (in Chinese)
[7] 杨膺白,梁贤威,郭辉,等.山羊尿中嘌呤衍生物排出规律的研究[J].黑龙江畜牧兽医,2011(1):64-66.
YANG Y B,LIANG X W,GUO H,et al.Study on the urinary excretion of purine derivatives on goat[J].Heilongjiang Animal Science and Veterinary Medicine,2011(1):64-66. (in Chinese)
[8] 马涛,刁其玉,邓凯东,等.日粮不同精粗比对肉羊氮沉积和尿嘌呤衍生物排出量的影响[J].中国畜牧杂志,2012,48(15):29-33.
MA T,DIAO Q Y,DENG K D.et al.Effect of different ratio of concentrate to roughage on N rentention and urinary excretion of purine derivatives in mutton sheep[J].Chinese Journal of Animal Science,2012,48(15):29-33. (in Chinese)
[9] 韦鸿战,莫彦.反刍动物瘤胃微生物蛋白合成的机理及影响因素进展[J].北京农业,2012(21):122.
WEI H Z,MO Y.Ruminant rumen microbial protein synthesis mechanism an dinfluencing factors of the progress[J].Beijing Agriculture,2012(21):122. (in Chinese)
[10] 赵江波,魏时来,赵明明,等.精料来源对肉羊营养物质消化率的影响及代谢能预测模型的建立[J].畜牧兽医学报,2016,47(11):2257-2265.
ZHAO J B,WEI S L,ZHAO M M,et al.Effect of concentrate source on nutrient digestibility and establishment of prediction model of metabolizable energy in mutton sheep[J].Acta Veterinaria et Zootechnica Sinica,2016,47(11):2257-2265. (in Chinese)
[11] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007.
ZHANG L Y.Feed analysis and quality inspection technology[M].3rd ed.Beijing:China Agricultural University Press,2007. (in Chinese)
[12] VAN SOEST P J, ROBERTSON J B, LEWIS B A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition[J]. J Dairy Sci, 1991, 74(10):3583-3597.
[13] CHEN X B,GOMES M J.Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives-an overview of the technical details[M].Bucksburn Aberdeen UK:International Feed Resources Unit,Rowett Research Institute,1995.
[14] GEORGE S K,VERMA A K,MEHRA U R,et al.Nitrogen utilization in goats fed various oil cakes[J].Archiva Zootechnica,2011,14(2):76-91.
[15] CHEN X B.Excretion of purine derivatives by sheep and cattle and its use for the estimation of absorbed microbial protein[D].Aberdeen:University of Aberdeen,1989.
[16] 孔祥浩,郭金双,朱晓萍,等.不同NDF水平肉羊日粮养分表观消化率研究[J].动物营养学报,2010,22(1):70-74.
KONG X H,GUO J S,ZHU X P,et al.Study on apparent nutrients digestibility of mutton sheep diets with different neutral detergent fiber levels[J].Chinese Journal of Animal Nutrition,2010,22(1):70-74. (in Chinese)
[17] 刘洁,刁其玉,赵一广,等.肉用绵羊饲料养分消化率和有效能预测模型的研究[J].畜牧兽医学报,2012,43(8):1230-1238.
LIU J,DIAO Q Y,ZHAO Y G,et al.Prediction of nutrient digestibility and energy concentrations using chemical compositions in meat sheep feeds[J].Acta Veterinaria et Zootechnica Sinica,2012,43(8):1230-1238. (in Chinese)
[18] 李志静,眭丹,周玉香.不同蛋白水平对舍饲滩羊消化代谢及血液生化指标的影响[J].中国畜牧杂志,2014,50(17):39-43.
LI Z J,XU D,ZHOU Y X.Effect of dietary protein level on nutrient digestion metabolism and serum biochemical indexes in Tan Sheep[J].Chinese Journal of Animal Science,2014,50(17):39-43. (in Chinese)
[19] 彭玉麟,贾志海,卢德勋,等.不同蛋白质水平的日粮对内蒙古白绒山羊消化代谢的影响[J].畜牧兽医学报,2002,33(4):321-326.
PENG Y L,JIA Z H,LU D X,et al.Effects of different protein levels on digestion and metabolism of Inner Mongolian White Cashmere goats[J].Acta Veterinaria et Zootechnica Sinica,2002,33(4):321-326. (in Chinese)
[20] 王波,柴建民,王海超,等.蛋白水平对早期断奶双胞胎湖羊公羔营养物质消化与血清指标的影响[J].畜牧兽医学报,2016,47(6):1170-1179.
WANG B,CHAI J M,WANG H C,et al.Effects of protein levels on nutrient digestion and metabolism and serum parameters of early-weaned male Hu twin lambs[J].Acta Veterinaria et Zootechnica Sinica,2016,47(6):1170-1179. (in Chinese)
[21] CASTILLO A R,KEBREAB E,BEEVER D E,et al.The effect of protein supplementation on nitrogen utilization in lactating dairy cows fed grass silage diets[J].J Anim Sci,2001,79(1):247-253.
[22] MARINI J C,VAN AMBURGH M E.Partition of nitrogen excretion in urine and the feces of Holstein replacement heifers[J].J Dairy Sci,2005,88(5):1778-1784.
[23] 楼灿,姜成钢,马涛,等.杜寒杂交繁殖母羊氮代谢和维持净蛋白质需要的研究[J].畜牧兽医学报,2014,45(6):943-952.
LOU C,JIANG C G,MA T,et al.Study of nitrogen metabolism and net protein requirement for maintenance of Dorper×Thin-tailed Han crossbred breeding ewes[J].Acta Veterinaria et Zootechnica Sinica,2014,45(6):943-952. (in Chinese)
[24] DENG K D,DIAO Q Y,JIANG C G,et al.Energy requirements for maintenance and growth of germ an mutton merino crossbred lambs[J].J Integr Agric,2013,12(4):670-677.
[25] ZOU C X,LIVELY F O,WYLIE A R G,et al.Estimation of the maintenance energy requirements,methane emissions and nitrogen utilization efficiency of two suckler cow genotypes[J].Animal,2016,10(4):616-622.
[26] JARDSTEDT M,HESSLE A,NØRGAARD P,et al.Feed intake and urinary excretion of nitrogen and purine derivatives in pregnant suckler cows fed alternative roughage-based diets[J].Livest Sci,2017,202:82-88.
[27] 楼灿,姜成钢,马涛,等.饲养水平对肉用绵羊空怀期和泌乳期营养物质消化代谢的影响[J].畜牧兽医学报,2015,46(3):407-415.
LOU C,JIANG C G,MA T,et al.Effects of different feeding levels on digestion and metabolism of meat ewes during non-pregnancy and lactation[J].Acta Veterinaria et Zootechnica Sinica,2015,46(3):407-415. (in Chinese)
[28] SENA J A B,VILLELA S D J,SANTOS R A,et al.Intake,digestibility,performance,and carcass traits of rams provided with dehydrated passion fruit (Passiflora edulis f.flavicarpa) peel,as a substitute of Tifton 85(Cynodon spp.)[J].Small Rumin Res,2015,129:18-24.
[29] HRISTOV A N,ETTER R P,ROPP J K,et al.Effect of dietary crude protein level and degradability on ruminal fermentation and nitrogen utilization in lactating dairy cows[J].J Anim Sci,2004,82(11):3219-3229.
[30] AGLE M,HRISTOV A N,ZAMAN S,et al.The effects of ruminally degraded protein on rumen fermentation and ammonia losses from manure in dairy cows[J].J Dairy Sci,2010,93(4):1625-1637.
[31] CHEN X B,MEJIA A T,KYLE D J,et al.Evaluation of the use of the purine derivative:creatinine ratio in spot urine and plasma samples as an index of microbial protein supply in ruminants:studies in sheep[J].J Agric Sci,1995,125(1):137-143.
[32] DIPU M T,GEORGE S K,SINGH P,et al.Measurement of microbial protein supply in Murrah buffaloes (Bubalus bubalis) using urinary purine derivatives excretion and PDC index[J].Asian-Australas J Anim Sci,2006,19(3):347-355.
[33] ZHOU J W,MI J D,DEGEN A A,et al.Urinary purine derivatives excretion,rumen microbial nitrogen synthesis and the efficiency of utilization of recycled urea in Tibetan and fine-wool sheep[J].Anim Feed Sci Technol,2017,227:24-31.
[34] JETANA T,SUTHIKRAI W,USAWANG S,et al.The effects of concentrate added to pineapple (Ananas comosus Linn.Mer.) waste silage in differing ratios to form complete diets,on digestion,excretion of urinary purine derivatives and blood metabolites in growing,male,Thai swamp buffaloes[J].Trop Anim Health Prod,2009,41(4):449-459.
[35] BELENGUER A,YAÑEZ D,BABER N H O,et al.Urinary excretion of purine derivatives and prediction of rumen microbial outflow in goats[J].Livest Prod Sci,2002,77(2-3):127-135.

Effect of Different Concentrates on Nutrient Digestibility and Excretion of Purine Derivatives in Urine of Mutton Sheep

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